Effect of bradykinin on prostaglandin production by human skin fibroblasts in culture
Journal Article
·
· Methods Enzymol.; (United States)
In studying the effect of bradykinin on prostaglandin formation in fibroblasts, two general types of assays have been employed. Radioimmunoassays were utilized to determine specific prostaglandins, PGI/sub 2/ and 6-keto-PGF, using tritium as radiolabel. A second procedure involved initial incubation of the fibroblasts with (/sup 14/C)arachidonate and identification and quantification of the metabolites by chromatographic methods. After radiolabelling of fibroblasts with (/sup 14/C)arachidonate, bradykinin was found to release radiolabel from membrane lipids approximately 2-fold. In the presence of bradykinin, there was a close coupling of phospholipase S/sub 2/ activity, arachidonate mobilization, and synthesis of a specific prostaglandin, PGI/sub 2/.
- OSTI ID:
- 6801694
- Journal Information:
- Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Dissociation of bradykinin-induced prostaglandin formation from phosphatidylinositol turnover in Swiss 3T3 fibroblasts: evidence for G protein regulation of phospholipase A/sub 2/
Stimulation of the synthesis of 15-hydroxyeicosatetraenoic acid (15-HETE) and 6-keto-prostaglandin F/sub 1. cap alpha. / (6-keto-PGF/sub 1. cap alpha. /) by cultured human umbilical veins
Very low levels of 6-keto-prostaglandin F /sub 1//sub. cap alpha. / in human plasma
Journal Article
·
Tue Sep 01 00:00:00 EDT 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:7253468
Stimulation of the synthesis of 15-hydroxyeicosatetraenoic acid (15-HETE) and 6-keto-prostaglandin F/sub 1. cap alpha. / (6-keto-PGF/sub 1. cap alpha. /) by cultured human umbilical veins
Conference
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:7189314
Very low levels of 6-keto-prostaglandin F /sub 1//sub. cap alpha. / in human plasma
Journal Article
·
Sun Feb 28 23:00:00 EST 1982
· J. Lab. Clin. Med.; (United States)
·
OSTI ID:5505028
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
BRADYKININ
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DATA
ENZYMES
ESTERASES
EXPERIMENTAL DATA
FIBROBLASTS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
LIPASE
LIPIDS
MEMBRANES
METABOLITES
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
RESPONSE MODIFYING FACTORS
SEPARATION PROCESSES
SKIN
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
BRADYKININ
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DATA
ENZYMES
ESTERASES
EXPERIMENTAL DATA
FIBROBLASTS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
LIPASE
LIPIDS
MEMBRANES
METABOLITES
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
RESPONSE MODIFYING FACTORS
SEPARATION PROCESSES
SKIN
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS