Distribution of progesterone receptor in the 20-day-old fetal mouse: an autoradiographic study with (/sup 125/I)progestin

Journal Article · Tue Nov 01 04:00:00 EST 1988 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-123-5-2382· OSTI ID:6801261

Shughrue, P J; Stumpf, W E; Sar, M

The distribution of progestin target sites in 20-day-old fetuses of estrogen-primed pregnant mice was investigated by thaw-mount autoradiography. Pregnant mice received a Silastic estradiol implant on day 17 and were ovariectomized on day 19 of pregnancy. Twenty-four hours after ovariectomy 10 prematurely delivered fetuses were each injected with 0.33 microgram/100 g BW (/sup 125/I)progestin (SA, 2200 Ci/mM). To show specificity of progestin localization two additional fetuses were each injected sc with 20 micrograms R5020, a synthetic progestin, 1 h before the injection of (/sup 125/I)progestin. The fetuses were frozen 2 h after injection of (/sup 125/I)progestin, sectioned, and processed for thaw-mount autoradiography. Cells with nuclear uptake and retention of radioactivity were observed in numerous tissues, including certain regions of the oral mucosa and developing teeth, esophagus, larynx, skin, mammary gland, skeletal muscle, kidney, and reproductive glands and ducts. Injection of unlabeled R5020 1 h before (/sup 125/I)progestin prevented nuclear concentration of radioactivity in all target tissues. The results indicate that progesterone receptors are expressed with a regional, cellular, and subcellular distribution in term fetal mouse tissues and suggest that progesterone is important to the growth and development of certain fetal tissues.

• Research Organization: Univ. of North Carolina, Chapel Hill (USA)
• OSTI ID: 6801261
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 123:5; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
FETUSES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KETONES
MAMMALS
MEMBRANE PROTEINS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANCY
PREGNANES
PROGESTERONE
PROTEINS
RADIOISOTOPES
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
TISSUE DISTRIBUTION
TISSUES
VERTEBRATES