Relationships between epitopes on IgE recognized by define monoclonal antibodies and by the Fc/sub epsilon/ receptor on basophils

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:6801161

Baniyash, M; Eshhar, Z; Rivnay, B

The present study investigated whether the sites on the Fc region on the IgE molecule, recognized by different anti-IgE monoclonal antibodies (mAb), are identical to those recognized by the Fc receptor (Fc,R). The anti-IgE mAb recognize different clusters of epitopes on the Fc region of IgE and could interfere to different degrees with the binding of IgE to mast cells and basophils, but still recognized cell-bound IgE. Analysis of the stoichiometry and affinity binding of /sup 125/I anti-IgE mAb Fab' to free IgE have revealed that anti-IgE mAb of one group (51.3) recognized three repetitive determinants on the IgE Fc portion, and another group (95.3) recognized only one determinant. When these stoichiometric studies were performed with cellbound IgE, it was found that only one of the sites recognized by 51.3 mAb was involved in the Fc/sub epsilon/R binding site. On the other hand, the site recognized by 95.3 mAb was not the Fc/sub epsilon/R binding site. Such findings establish mAb 51.3 as a useful tool for isolating the IgE peptides involved in the binding site to the receptor.

• Research Organization: Weizmann Institute of Science, Rohovat, Israel
• OSTI ID: 6801161
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
MAST CELLS
MATERIALS
MEMBRANE PROTEINS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RECEPTORS
SOMATIC CELLS
TRACER TECHNIQUES