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Properties of a second epitope of the murine Fc receptor for aggregated IgG

Journal Article · · J. Immunol.; (United States)
OSTI ID:5436224
; ; ; ;
The murine macrophage and lymphocyte Fc receptor for aggregated IgC(Fc..gamma..R) has previously been characterized by using the anti-Fc..gamma..R monoclonal antibody (mAb), 2.4G2. In the studies presented here, the authors describe a new mAb, 6B7C, that defines a second epitope of the Fc..gamma..R. The tissue distribution of the 6B7C epitope is coincident with the 2.4G2 epitope. However, only the 2.4G2 epitope is accessible to mAb binding on intact primary macrophages or lymphocytes. The 6B7C epitope is not detectable on primary macrophages or lymphocytes but is exposed on a portion of B lymphocyte Fc..gamma..R after activation by lipopolysaccharide and on some tumor cell lines. The expression of the 6B7C epitope on the surface of B lymphoblasts and tumor cell lines seems to correlate with their ability to release soluble Fc..gamma..R. The 6B7C mAb has the advantage that it reacts with native as well as denatured receptor and therefore can be used for techniques such as immunoblotting. The binding assay was done using /sup 125/I-labeled Ig.
Research Organization:
Rockefeller Univ., New York, NY
OSTI ID:
5436224
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:12; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GLOBULINS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
RECOMBINANT DNA
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES