Effect of ethanol on (/sup 3/H)dopamine release in rat nucleus accumbens and striatal slices

Journal Article · Sun May 01 04:00:00 EDT 1988 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF01268885· OSTI ID:6800122

Russell, V A; Lamm, M C; Taljaard, J J

Ethanol (10-200 mM) transiently increased tritium overflow from superfused rat nucleus accumbens slices previously incubated with (/sup 3/H)dopamine (DA) and (/sup 14/C)choline. The effect was greater in striatal tissue and did not appear to be a non-specific membrane effect since (/sup 14/C)acetylcholine (ACh) release was not affected. Lack of antagonism by picrotoxin suggested that gamma-aminobutyric acid (GABA) receptors were not involved. Calcium was not a requirement and the DA uptake blocker, nomifensine, was without effect. Ethanol appeared to be causing (/sup 3/H)DA release into the cytoplasm. K+ -stimulated release of (/sup 3/H)DA and (/sup 14/C)ACh from nucleus accumbens and striatal slices was not affected. Clonidine-mediated inhibition of the K+-evoked release of (/sup 3/H)DA remained unaltered. Ethanol attenuated the isoproterenol-induced enhancement of (/sup 3/H)DA release. Ethanol therefore appeared to interact with components of the DA terminal causing a transient increase in the release of neurotransmitter without impairing K+-evoked release but apparently interfering with the isoproterenol-induced effect.

• Research Organization: Univ. of Stellenbosch, Tygerberg Hospital (South Africa)
• OSTI ID: 6800122
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 13:5; ISSN NERED
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLCHOLINE
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHOLINE
CYTOPLASM
DOPAMINE
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ESTERS
ETHANOL
HYDROXY COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PHENOLS
POLYPHENOLS
QUATERNARY COMPOUNDS
RATS
RODENTS
SECRETION
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES