DNA repair and induction of plasminogen activator in human fetal cells treated with ultraviolet light. [Development associated changes]

Journal Article · Wed Feb 29 23:00:00 EST 1984 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/5.3.357· OSTI ID:6800096

Ben-Ishai, R; Sharon, R; Rothman, M ^[1]; Miskin, R ^[2]

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1. Technion-Israel Inst. of Tech., Haifa. Dept. of Biology
2. Weizmann Inst. of Science, Rehovoth (Israel). Dept. of Biochemistry

Human fetal fibroblasts have been tested for development associated changes in DNA repair by utilizing nucleoid sedimentation as an assay for excision repair. Among skin fibroblasts the rate of excision repair was significantly higher in non-fetal cells than in fibroblasts derived from an 8 week fetus. Skin fibroblasts derived at 12 week gestation were more repair proficient than at 8 weeks. However, they exhibited a lower rate of repair than non-fetal cells. Enhancement of protease plasminogen activator (PA) was higher after u.v. irradiation in skin fibroblasts derived at 8 weeks than at 12 weeks gestation and was absent in non-fetal skin fibroblasts. Excision repair and PA inducibility depended on the tissue of origin in addition to gestational stage, as shown for skin and lung fibroblasts from the same 12 week fetus. The sedimentation velocity of nucleoids, prepared from unirradiated fibroblasts, in neutral sucrose gradients with or without ethidium bromide, indicated the presence of DNA strand breaks in fetal cells. It is proposed that reduced DNA repair in fetal cells may result from alterations in DNA supercoiling, and that persistent DNA strand breaks enhance transcription of PA gene(s).

• OSTI ID: 6800096
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 5:3; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
FETUSES
FIBROBLASTS
HYDROLASES
LUNGS
MAMMALS
MAN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RESPIRATORY SYSTEM
SERINE PROTEINASES
SKIN
SOMATIC CELLS
TRYPSIN
ULTRAVIOLET RADIATION
VERTEBRATES