Distribution of radiolabeled human and mouse monoclonal IgM antibodies in murine models

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · J. Nucl. Med.; (United States)

OSTI ID:6799507

Halpern, S E; Hagan, P L; Chen, A; Birdwell, C R; Bartholomew, R M; Burnett, K G; David, G S; Poggenburg, K; Merchant, B; Carlo, D J

The distribution and kinetics of six human and one murine monoclonal IgM antibodies (MoAb) were studied in BALB/c mice. Labeling was with /sup 111/In, /sup 75/Se, and /sup 125/I. The monomers and pentamers of certain MoAbs were studied. Human distribution studies were also performed. The serum containing (/sup 111/In)MoAb was obtained from one of the patients 24 hr after administration and injected into mice which were then killed and assayed for /sup 111/In distribution. In general, the (/sup 75/Se) and (/sup 111/In)MoAbs had distribution and kinetic patterns that were similar while the /sup 125/I-labeled MoAbs dehalogenated after 4 hr. Monomers and pentamers had highly similar distributions suggesting that the distribution of IgMs may be based on factors other than molecular size. The murine IgM showed a somewhat different distribution in mice than did human IgMs. Serum from the patient containing (/sup 111/In)MoAb had a distribution in mice similar to that of the patient with high liver and gastrointestinal uptake. The human imaging indicates that it is possible to target tumor with human IgM MoAbs, but significant problems remain in regard to their clinical use.

• Research Organization: Veterans Administration Medical Center, San Diego, CA (USA)
• OSTI ID: 6799507
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 29:10; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IMAGE PROCESSING
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
PRIMATES
PROCESSING
RADIOISOTOPES
REACTION KINETICS
RODENTS
SELENIUM 75
SELENIUM ISOTOPES
SPECIFICITY
TISSUE DISTRIBUTION
TRACER TECHNIQUES
USES
VERTEBRATES