Renal tubular secretion of glutathione (GSH)
The rapid turnover of renal GSH may require its secretion into the tubular lumen. Renal clearance of plasma GSH was measured in rats anesthetized with Inactin and infused with (/sup 3/H)inulin. Renal ..gamma..-glutamyltranspeptidase (..gamma..GT) was then inactivated (> 97%) by infusion of acivicin and samples were collected for 6-7 h. By 4.5 h arterial and urinary GSH increased from 5..mu..M and 1.3 n mol/h to 23 ..mu..M and 2400-7000 nmol/h, respectively. The ratio of urinary GSH to filtered load increased from < 0.01 to 0.7-2.6. When renal GSH was decreased to 30% of normal by pretreating rats with buthionine sulfoximine (BSO), the subsequent inactivation of ..gamma..GT caused only a slight increase in arterial GSH and urinary GSH increased to only 400-600 nmol/h (60-70% of filtered load). The amount of GSH filtered by the kidney was reduced by initially treating a rat with acivicin and 3 h later infusing purified ..gamma..GT (0.2 mg/h) to degrade plasma GSH. Just before infusion of ..gamma..GT, arterial GSH was 23 ..mu..M and urinary GSH was equal to 90% of the filtered load. At 1 h after infusion of ..gamma..GT, arterial GSH decreased to 0.3 ..mu..M, whereas urinary GSH remained elevated (1200-1800 nmol/h) and now equalled 10-20 times the filtered load. When similar experiments were carried out in BSO treated rats, maximal urinary GSH was reduced to 200 nmol/h, a value that was still 10 times the filtered load. Therefore, secreted GSH constitutes a significant portion of the GSH that is normally catabolized within the tubular lumen.
- Research Organization:
- Univ. of Pittsburgh School of Medicine, PA
- OSTI ID:
- 6797591
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transepithelial transport of glutathione in isolated perfused small intestine
Glutathione (GSH) production is increased in copper deficient isolated hepatocytes and inhibition of GSH synthesis decreases intracellular cholesterol
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CARBOHYDRATES
CLEARANCE
DRUGS
ENZYME ACTIVITY
ENZYMES
EXCRETION
GLUTATHIONE
HYDROLASES
INULIN
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PEPTIDES
POLYPEPTIDES
POLYSACCHARIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RENAL CLEARANCE
RODENTS
SACCHARIDES
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUBULES
VERTEBRATES