Glutathione (GSH) production is increased in copper deficient isolated hepatocytes and inhibition of GSH synthesis decreases intracellular cholesterol
- Colorado State Univ., Ft. Collins (United States)
Thirty male weanling Sprague Dawley rats were assigned to three groups of 10 animals each and fed AIN based copper deficient ad libitum, copper control meal fed and copper deficient meal fed diets. 12h meals were provided. Beginning on day 54 animals were used to prepare isolated hepatocytes, following a 4h meal, by portal vein collagenase infusion. Washed isolated hepatocytes were incubated in Krebs Henseleit bicarbonate buffer pH 7.4 under 95/5 O{sub 2}/CO{sub 2} at 37{degree} with 2.5 mM Ca, 0.75% gelatin and 10 mM glucose, either with or without 1 mM buthionine sulfoximine (BSO) a specific inhibitor of GSH synthesis. Hepatocyte viability was assessed by trypan blue exclusion. Copper deficiency significantly increased GSH production by 30-50% at 1.5 and 3 h, with no changes in GSSG. GSH production was inhibited by BSO which also significantly inhibited intracellular cholesterol appearance at 3 h. The intracellular (GHS){sup 2}/GSSG at 3 hr showed a significant correlation with cholesterol appearance rate at 3 h. Copper deficiency increases hepatocyte GSH production. Inhibition of GSH production is correlated with decreased cholesterol appearance.
- OSTI ID:
- 5202679
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
The toxic effects, GSH depletion and radiosensitivity by BSO on retinoblastoma
Depletion of intracellular GSH and NPSH by buthionine sulfoximine and diethyl maleate: factors that influence enhancement of aerobic radiation response
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CHOLESTEROL
COPPER
DRUGS
ELEMENTS
ENZYME INHIBITORS
GLUTATHIONE
HYDROXY COMPOUNDS
INHIBITION
LIVER CELLS
MAMMALS
METABOLISM
METALS
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SOMATIC CELLS
STEROIDS
STEROLS
SYNTHESIS
TRANSITION ELEMENTS
VERTEBRATES