The distribution and metabolism of Tc-/sup 99m/ labeled low density lipoprotein in human subjects
The distribution of Tc-99m labeled low density lipoprotein (LDL) was determined in 8 human subjects with documented atherosclerosis involving the carotid or coronary arteries. Autologous LDL was isolated by ultracentrifugation, labeled with 370 to 740 MBq of Tc-99m using a dithionite reduction technique, purified on a column of Sephadex G-50 and administered intravenously. Serial images were recorded over a 28 hour interval using a large field scanning scintillation camera and a dedicated computer. In parallel with the imaging, serial blood samples were obtained to define blood clearance. The data were displayed to identify sites of focal tracer concentration in the arterial tree and to define changes in heart and liver activity. The radiopharmaceutical had excellent blood pool retention, with remarkable contrast between blood pool and soft tissues throughout the imaging interval. The half-time of clearance of LDL from blood was 29.66 +- 8.98h which was in good agreement with the value of 27.67 +- 6.72h determined by imaging.
- Research Organization:
- New England Deaconess Hospital, Boston, MA
- OSTI ID:
- 6795514
- Report Number(s):
- CONF-850611-
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ARTERIES
ARTERIOSCLEROSIS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CAROTID ARTERIES
CHEMICAL PREPARATION
CHROMATOGRAPHY
CORONARIES
CORRELATIONS
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DRUGS
FUNCTIONS
GEL PERMEATION CHROMATOGRAPHY
GLANDS
HEART
HOURS LIVING RADIOISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MAN
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
RETENTION FUNCTIONS
SCINTISCANNING
SEPARATION PROCESSES
SYNTHESIS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
UPTAKE
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES