The effects of elongation on the permeability and conduction characteristics of peripheral nerve

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6794299

Beel, J A

The present studies analyze the effects of quasi-static tensile loading on the conduction and permeability characteristics of mouse sciatic nerve. Mechanical evaluations of nerve and roots were made via force and photographic records taken during elongation. Using strains to the proportional limit, functional properties of nerve were investigated. Recording of compound action potentials (CAP) during stretch yielded reductions in CAP velocity, slope, and area. Increments were observed in rise time, fall time, and duration. Vascular permeability was investigated for identical strains. SDS-PAGE analyses of nerve proteins yielded minimal changes in albumin concentration with stretch. Paper chromatography analyses of endoneurial fluid showed increments in amino acid concentrations during stretch. Perineurium permeability was studied using radiolabel techniques. Nerve stretch resulted in increased {sup 14}C-dextran accumulation at higher strains with additional accumulation during relaxation. Perineurial permeability increases were attributed to expansion of perineurial holes during stretch.

• Research Organization: Colorado Univ., Boulder, CO (USA)
• OSTI ID: 6794299
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL STRESS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
DYNAMIC FUNCTION STUDIES
ELECTROPHORESIS
ELONGATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MICE
NERVES
NERVOUS SYSTEM
PERMEABILITY
RODENTS
SCIATIC NERVE
TRACER TECHNIQUES
VERTEBRATES