In vitro cytotoxicity of human recombinant tumor necrosis factor alpha in association with radiotherapy in a human ovarian carcinoma cell line
Journal Article
·
· Gynecologic Oncology; (USA)
- Univ. of California Irvine Medical Center, Orange (USA)
It has been speculated that tumor necrosis factor alpha (TNF-alpha) may decrease the cytotoxicity of radiotherapy by increasing the scavenging of toxic superoxide radicals. Because of the possible clinical implications, the cytotoxicity of TNF-alpha in combination with radiotherapy (RT) was compared with that of RT alone in a human ovarian cancer cell line. NIH:OVCAR-3 cells were incubated with TNF-alpha at 10.0, 1.0, 0.1, and 0.01 microgram/ml. Plates were divided into two groups; one received 150 cGy of radiotherapy and the other received no further therapy. Seventy-two hours later, supernatants were aspirated and viable cells were stained with a 1% solution of crystal violet. Survival of cells treated with RT plus TNF-alpha was expressed as a percentage of surviving irradiated controls. Analysis of results revealed minimal additive cell killing effect between TNF-alpha and radiotherapy at all concentrations of tumor necrosis factor, with the greatest difference noted in the group treated with 10 micrograms/ml TNF-alpha. A continued radiotherapy dose-response study with TNF-alpha showed a similar additive, not radioprotective, effect. This may have implication as a potentiator of RT in some human tumors.
- OSTI ID:
- 6791317
- Journal Information:
- Gynecologic Oncology; (USA), Journal Name: Gynecologic Oncology; (USA) Vol. 38:2; ISSN 0090-8258; ISSN GYNOA
- Country of Publication:
- United States
- Language:
- English
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550603* -- Medicine-- External Radiation in Therapy-- (1980-)
560151 -- Radiation Effects on Animals-- Man
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CARCINOMAS
CELL KILLING
DISEASES
DOSE-RESPONSE RELATIONSHIPS
FEMALE GENITALS
GONADS
GROWTH FACTORS
MAMMALS
MAN
MEDICINE
MITOGENS
NEOPLASMS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANS
OVARIES
PRIMATES
PROTEINS
RADICALS
RADIOLOGY
RADIOSENSITIVITY EFFECTS
RADIOTHERAPY
SUPEROXIDE RADICALS
SURVIVAL TIME
THERAPY
VERTEBRATES
560151 -- Radiation Effects on Animals-- Man
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CARCINOMAS
CELL KILLING
DISEASES
DOSE-RESPONSE RELATIONSHIPS
FEMALE GENITALS
GONADS
GROWTH FACTORS
MAMMALS
MAN
MEDICINE
MITOGENS
NEOPLASMS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANS
OVARIES
PRIMATES
PROTEINS
RADICALS
RADIOLOGY
RADIOSENSITIVITY EFFECTS
RADIOTHERAPY
SUPEROXIDE RADICALS
SURVIVAL TIME
THERAPY
VERTEBRATES