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Effect of gamma radiation on interaction between DNA and nonhistone proteins of chromatin and karyolymph. [In vitro]

Journal Article · · Radiobiology (USSR) (Engl. Transl.); (United States)
OSTI ID:6788766
;

Interaction of nonhistone proteins of chromatin (NPC) and proteins of karyolymph (PK) with DNA were studied by two methods: fixation of protein-/sup 14/C-DNA complexes on nitrocellulose filters and chromatography of /sup 32/P-labeled proteins on DNA-containing polyacrylamide-agarose columns. It was shown that irradiated DNA binds somewhat more NPC and PK and much more stably than native DNA. The effect is enhanced with increase in dosage, and it is related to appearance of denatured sites in the DNA. Irradiation of NPC does not have an appreciable effect on their capacity to bind DNA; however, there is impairment of specific interaction between part of the NPC and homologous DNA. The possible role of radiation impairment of interaction between DNA and NPC and PK is discussed, with regard to change in structure and function of chromatin.

Research Organization:
Inst. of Biological Physics, Pushchino, USSR
OSTI ID:
6788766
Journal Information:
Radiobiology (USSR) (Engl. Transl.); (United States), Journal Name: Radiobiology (USSR) (Engl. Transl.); (United States) Vol. 18:1; ISSN RADBA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BINDING ENERGY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL NUCLEI
CESIUM 137
CESIUM ISOTOPES
CHROMATIN
DAYS LIVING RADIOISOTOPES
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENERGY
GAMMA RADIATION
IN VITRO
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACID DENATURATION
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
TRACER TECHNIQUES
TRANSCRIPTION
YEARS LIVING RADIOISOTOPES