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Title: Effects of gentamicin and monomer on bone. An in vitro study

Abstract

Gentamicin-loaded bone cement is used with increasing frequency in primary and revision arthroplasty. Considering the high local concentration and the well-known toxic effect of gentamicin on the kidney, a similar inhibiting effect on bone tissue might be expected. In a series of in vitro studies using paired mouse calvaries cultured for 2 days, the authors found a dose-dependent decrease in the release of previously incorporated calcium-45 (UVCa) or tritiated proline and a decrease in alkaline phosphatase activity. In combination with methylmethacrylate, a small additional reduction in UVCa release and a marked decrease in alkaline phosphatase activity were recorded. These results indicate that released gentamicin and monomer from antibiotic-supplemented bone cement depresses bone turnover and might thus play an important part in the pathogenesis of loosening.

Authors:
;
Publication Date:
Research Org.:
Univ. Hospital of Copenhagen (Denmark)
OSTI Identifier:
6786562
Alternate Identifier(s):
OSTI ID: 6786562
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Arthroplasty; (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALKALINE PHOSPHATASE; ENZYME ACTIVITY; ANTIBIOTICS; BIOLOGICAL EFFECTS; BONE CELLS; CELL DIFFERENTIATION; CALCIUM 45; DOSE-RESPONSE RELATIONSHIPS; IN VITRO; MICE; PROLINE; SKELETON; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALINE EARTH ISOTOPES; AMINES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; ANTI-INFECTIVE AGENTS; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CALCIUM ISOTOPES; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; ESTERASES; EVEN-ODD NUCLEI; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROLASES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHOSPHATASES; PYRROLES; PYRROLIDINES; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques

Citation Formats

Pedersen, J.G., and Lund, B. Effects of gentamicin and monomer on bone. An in vitro study. United States: N. p., 1988. Web. doi:10.1016/S0883-5403(88)80011-1.
Pedersen, J.G., & Lund, B. Effects of gentamicin and monomer on bone. An in vitro study. United States. doi:10.1016/S0883-5403(88)80011-1.
Pedersen, J.G., and Lund, B. Fri . "Effects of gentamicin and monomer on bone. An in vitro study". United States. doi:10.1016/S0883-5403(88)80011-1.
@article{osti_6786562,
title = {Effects of gentamicin and monomer on bone. An in vitro study},
author = {Pedersen, J.G. and Lund, B.},
abstractNote = {Gentamicin-loaded bone cement is used with increasing frequency in primary and revision arthroplasty. Considering the high local concentration and the well-known toxic effect of gentamicin on the kidney, a similar inhibiting effect on bone tissue might be expected. In a series of in vitro studies using paired mouse calvaries cultured for 2 days, the authors found a dose-dependent decrease in the release of previously incorporated calcium-45 (UVCa) or tritiated proline and a decrease in alkaline phosphatase activity. In combination with methylmethacrylate, a small additional reduction in UVCa release and a marked decrease in alkaline phosphatase activity were recorded. These results indicate that released gentamicin and monomer from antibiotic-supplemented bone cement depresses bone turnover and might thus play an important part in the pathogenesis of loosening.},
doi = {10.1016/S0883-5403(88)80011-1},
journal = {J. Arthroplasty; (United States)},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}