Ethanol inhibits human bone cell proliferation and function in vitro
- University of Washington, Seattle (USA)
The direct effects of ethanol on human bone cell proliferation and function were studied in vitro. Normal human osteoblasts from trabecular bone chips were prepared by collagenase digestion. Exposure of these osteoblasts to ethanol in concentrations of 0.05% to 1% for 22 hours induced a dose-dependent reduction in bone cell DNA synthesis as assessed by incorporation of 3H-thymidine. After 72 hours of ethanol exposure in concentrations of 0.01% to 1%, protein synthesis as measured by 3H-proline incorporation into trichbroacetic acid (TCA)-precipitable material was reduced in a dose-dependent manner. Human bone cell protein concentrations and alkaline phosphatase total activity were significantly reduced after exposure to 1% ethanol for 72 hours, but not with lower concentrations of ethanol. This reduction in osteoblast proliferation and activity may partially explain the development of osteopenia in humans consuming excessive amounts of ethanol.
- OSTI ID:
- 5185723
- Journal Information:
- Metabolism, Clinical and Experimental; (United States), Journal Name: Metabolism, Clinical and Experimental; (United States) Vol. 40:6; ISSN METAA; ISSN 0026-0495
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE PHOSPHATASE
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZINES
AZOLES
BIOLOGICAL EFFECTS
BONE CELLS
CARBOXYLIC ACIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ETHANOL
ETIOLOGY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MAN
MITOTIC INDEX
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHATASES
PRIMATES
PROLINE
PROTEINS
PYRIMIDINES
PYRROLES
PYRROLIDINES
RIBOSIDES
SKELETAL DISEASES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES