Induction of metallothionein-I and metallothionein-II in rats by cadmium and zinc

Journal Article · Tue Mar 31 23:00:00 EST 1987 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(87)90005-6· OSTI ID:6786115

Lehman-McKeeman, L D; Klaassen, C D

In this study, induction of the isoforms of metallothionein (MT) by Cd and Zn was determined with a high-performance liquid chromatography method. Rats were injected (sc) with Cd (1-100 mumol/kg) or Zn (100-10,000 mumol/kg) and concentrations of MT-I and MT-II in liver, kidney, and pancreas were measured 24 hr later. In control rats, only MT-II was detected in liver, whereas both forms were detected in kidney. In liver, Cd treatment increased MTs at all dosages tested such that concentrations of MT-I and MT-II were approximately equal. Zn (100-1000 mumol/kg) increased concentrations of MT-II about three times higher than MT-I, but higher dosages increased both isoforms to similar levels. Time-course experiments indicated that, at all times after Cd administration (30 mumol/kg), concentrations of MT-I and MT-II in liver were similar. However, with Zn treatment (1000 mumol/kg), MT-II levels were three times higher than MT-I at all times after 6 hr. In kidney, no differences in induction of the isoforms was observed following Cd or Zn treatment. In pancreas, Zn (300-10,000 mumol/kg) induced MT-I and MT-II to similar levels. Thus, in liver and kidney, Cd induced MT-I and MT-II similarly over a wide range of dosages. However, Zn treatment preferentially increased hepatic MT-II at low dosages but induced MT-I and MT-II to similar levels at high dosages. In contrast, Zn induced MT-I and MT-II to similar levels in kidney and pancreas.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 6786115
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 2; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BODY
CADMIUM
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DIGESTIVE SYSTEM
DISTRIBUTION
ELEMENTS
ENDOCRINE GLANDS
GLANDS
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
METALS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PROTEINS
RATS
RODENTS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
VERTEBRATES
ZINC