Effects of cadmium and zinc on tissue levels of metallothionein. [Rats]
Although the induced synthesis of metallothionein (MT) after exposure to certain metals has been known for some time, there is little information on the quantitation of MT in various tissues. In this study, tissue MT concentrations were measured by a modified Cd-saturation method in tissues of adult male rats after injection of different metal salts. There were differences in tissue levels of MT, depending on the injected metals. Of all the metals studied, Cd/sup 2 +/ was the most effective element in increasing MT concentrations in liver, kidney, pancreas and small intestine. The highest increase in tissue MT concentrations after CdCl/sub 2/ injection was found in the liver, while the pancreas contained the highest MT level after ZnSO/sub 4/ injection. Co and Ni salts increased MT levels in both liver and kidney, while Mn and Ca increased MT levels only in liver. A direct correlation between tissue MT levels and Cd or Zn concentration was observed in most of the tissues after injection of CdCl/sub 2/ or ZnSO/sub 4/. Although there was no positive relationship between tissue levels of MT and tissue Mn and Ni concentrations, the increase in hepatic Zn after injection of these metals was related to hepatic MT levels. The tissue distribution of injected Cd/sup 2 +/ in control adult rats and Zn-deficient was similar. However, there was no increase in pancreatic MT levels in Zn-deficient rats after injection of CdCl/sub 2/. The high concentration of MT in pancreas after ZnSO/sub 4/ injection in adult rats and the inability of the pancreas to synthesize MT in Zn-deficient rats suggest that the induction of pancreatic MT synthesis is sensitive to Zn status. Thus, injection of different metals results in quantitative variations of tissue MT concentrations. 30 references, 1 figure, 5 tables.
- Research Organization:
- Kobe-Gokiun Univ., Japan
- OSTI ID:
- 5975379
- Journal Information:
- Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 54; ISSN EVHPA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CADMIUM
CATIONS
CHARGED PARTICLES
COBALT
DATA
DIGESTIVE SYSTEM
DISTRIBUTION
ELEMENTS
ENDOCRINE GLANDS
EXPERIMENTAL DATA
GASTROINTESTINAL TRACT
GLANDS
INFORMATION
INTESTINES
IONS
KIDNEYS
LIVER
MAMMALS
MANGANESE
MEASURING METHODS
METALLOPROTEINS
METALLOTHIONEIN
METALS
NICKEL
NUMERICAL DATA
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PROTEINS
RATS
RODENTS
SMALL INTESTINE
SYNTHESIS
TISSUE DISTRIBUTION
TRANSITION ELEMENTS
VARIATIONS
VERTEBRATES
ZINC