On-line DNA analysis system with rapid thermal cycling

Patent · 10 August 1999

OSTI ID:678584

Swerdlow, H P; Wittwer, C T

This application describes an apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column. 6 figs.

• Research Organization: Oak Ridge Associated Universities
• Sponsoring Organization: USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
• DOE Contract Number: AC05-76OR00033
• Assignee: Univ. of Utah Research Foundation, Salt Lake City, UT (United States)
• Patent Number(s): US 5,935,522/A/
• Application Number: PAN: 8-885,625; CNN: Contract 5P30HG00199
• OSTI ID: 678584
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MATERIALS
CHROMATOGRAPHY
DESIGN
ELECTROPHORESIS
LASERS
SAMPLE PREPARATION