skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: On-line DNA analysis system with rapid thermal cycling

Abstract

An apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column.

Inventors:
 [1];  [1]
  1. Salt Lake City, UT
Publication Date:
Research Org.:
Oak Ridge Associated Univ., Oak Ridge, TN (United States)
OSTI Identifier:
872436
Patent Number(s):
US 5935522
Assignee:
University of Utah Research Foundation (Salt Lake City, UT)
DOE Contract Number:  
AC05-76OR00033
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
on-line; dna; analysis; rapid; thermal; cycling; apparatus; particularly; suited; subjecting; biological; samples; sample; preparation; tasks; subsequent; analytical; techniques; chromatography; device; including; injection; means; pump; column; addition; contains; capillary; electrophoresis; consisting; inlet; outlet; applying; voltage; differential; migration; species; effluent; liquid; passes; tee; structure; loading; desired; supply; activated; precise; polarity; specific; duration; diverted; flowing; stream; laser; induced; fluorescence; detector; preferably; analyze; products; separated; biological samples; liquid chromatograph; sample preparation; capillary column; voltage supply; particularly suited; capillary electrophoresis; thermal cycling; device including; liquid chromatography; laser induced; rapid thermal; chromatography column; flowing stream; analytical techniques; injection means; fluorescence detector; induced fluorescence; biological sample; electrophoresis device; analytical technique; /422/73/204/210/435/436/

Citation Formats

Swerdlow, Harold P, and Wittwer, Carl T. On-line DNA analysis system with rapid thermal cycling. United States: N. p., 1999. Web.
Swerdlow, Harold P, & Wittwer, Carl T. On-line DNA analysis system with rapid thermal cycling. United States.
Swerdlow, Harold P, and Wittwer, Carl T. 1999. "On-line DNA analysis system with rapid thermal cycling". United States. https://www.osti.gov/servlets/purl/872436.
@article{osti_872436,
title = {On-line DNA analysis system with rapid thermal cycling},
author = {Swerdlow, Harold P and Wittwer, Carl T},
abstractNote = {An apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column.},
doi = {},
url = {https://www.osti.gov/biblio/872436}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

Works referenced in this record:

Allelic discrimination by nick-translation PCR with fluorgenic probes
journal, August 1993


Automated polymerase chain reaction in capillary tubes with hot air
journal, January 1989


And the winner: Cetus does own PCR
journal, March 1991


PCR in a silicon microstructure
journal, September 1994


PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
journal, March 1994


Analytical strategies for the use of DNA probes
journal, February 1988


Detection of rare mRNAs via quantitative RT-PCR
journal, August 1992


Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
journal, January 1997


Biotech nightmare: does Cetus own PCR?
journal, February 1991


Molecular Beacons: Probes that Fluoresce upon Hybridization
journal, March 1996


Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus
journal, May 1993


Sensitive fluorescence-based thermodynamic and kinetic measurements of DNA hybridization in solution
journal, March 1993


Simultaneous Amplification and Detection of Specific DNA Sequences
journal, April 1992


Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides.
journal, July 1995


Minimizing the time required for DNA amplification by efficient heat transfer to small samples
journal, May 1990


Effect of heat denaturation of target DNA on the PCR amplification
journal, January 1993


Real-Time Monitoring of DNA Manipulations Using Biosensor Technology
journal, January 1995


The LightCycler TM : A Microvolume Multisample Fluorimeter with Rapid Temperature Control
journal, January 1997


A Personal Technology Transfer Effort in DNA Diagnostics
journal, November 1994


Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
journal, August 1991


Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
journal, September 1993


Digoxigenin-Labeled Probes Amplified from Genomic DNA Detect T-Cell Gene Rearrangements
journal, May 1992


Cyanine dye labeling reagents containing isothiocyanate groups
journal, January 1989


Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction
journal, September 1993


Lyme Disease
journal, January 1990


Mutation in blood coagulation factor V associated with resistance to activated protein C
journal, May 1994


Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction
journal, February 1997


Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer.
journal, December 1988


Advanced PCR
journal, August 1994


Fully Automated DNA Reaction and Analysis in a Fluidic Capillary Instrument
journal, March 1997


The incredible shrinking laboratory
journal, April 1995


Real time kinetics of restriction endonuclease cleavage monitored by fluorescence resonance energy transfer
journal, January 1994