Pilot scale production of cellulolytic enzymes by Trichoderma reesei
The French substitute fuels program aims at the substitution of part of gasoline by methanol. In order to avoid phase separation of the gasoline-methanol blend, a cosolvant has to be added; one of the most efficient cosolvants is the mixture of acetone and butanol produced by anaerobic acetone-butanol fermentation. The Institut Francais du Petrole is thus implementing a research and development program on the production of acetone butanol from biomass, either sugar crops (fodder beets and Jerusalem artichoke) or lignocellulosic (corn stover and wheat straw). Production of sugars from lignocellulosics is a major part of this program. The enzymatic hydrolysis route, based on Trichoderma reesei cellulolytic enzymes, has been chosen since it does not cause any degradation of C/sub 5/ sugars which are good substrates of the acetone butanol fermentation. Efficient and cheap large-scale production of cellulolytic enzymes is thus a key step in this process. This paper reports on production of cellulases by Trichoderma reesei in a 3-m/sup 3/ pilot fermentor under conditions which should facilitate the scaling-up of the process. 7 references, 2 figures, 2 tables.
- Research Organization:
- Institut Francais du Petrole, Rueil-Malmaison
- OSTI ID:
- 6783074
- Report Number(s):
- CONF-830567-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Vol. 13; Conference: 5. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 10 May 1983
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
CELLULASE
BIOSYNTHESIS
ENZYMES
TRICHODERMA VIRIDE
BATCH CULTURE
XYLANASE
BIOREACTORS
ENZYME ACTIVITY
FERMENTATION
PROCESS DEVELOPMENT UNITS
BIOCONVERSION
FUNCTIONAL MODELS
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
PLANTS
SYNTHESIS
TRICHODERMA
550700* - Microbiology
550200 - Biochemistry
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)