In vivo effect of staphylococcal enterotoxin A on peripheral blood lymphocytes

Journal Article · Tue May 01 04:00:00 EDT 1984 · Infect. Immun.; (United States)

OSTI ID:6782999

Zehavi-Willner, T; Shenberg, E; Barnea, A

Staphylococcal enterotoxin A (SEA) administration to monkeys produced an initial lymphocytic leukopenia lasting approximately 24 h. Lymphocytes isolated from blood circulation (PBL) during this stage had normal or decreased (/sup 3/H)thymidine incorporating activity. After 48 h, however, a significant increase (five- to sixfold) in (/sup 3/H)thymidine incorporating activity into PBL was apparent. The peak of incorporating activity (seven- to eightfold) was reached 3 to 4 days after SEA administration, followed by a gradual decline, reaching the baseline after 2 weeks. The increased levels of (/sup 3/H) thymidine incorporation in PBL were concomitant with the conversion of lymphopenia into lymphocytosis, accompanied by the release of many immature cells into the circulation. Lymphocytes isolated 24 h after SEA administration in vivo did not respond to the mitogenic action of SEA in vitro. Lymphocytes isolated at later stages after SEA challenge were fully activated by toxin. From a series of studies, it was concluded that SEA administered to monkeys caused, during the initial 24 h, the removal of a great proportion of lymphocytes from the circulation, followed by the release of new immature cells with augmented DNA synthesis activity. The lymphocytic leukocytosis state declined gradually and reached normal levels between 3 and 4 weeks after the SEA challenge. The biological implications of the hematological changes occurring after SEA challenge in vivo are discussed.

• Research Organization: Department of Microbiology, Israel Institute for Biological Research, Ness-Ziona, Israel
• OSTI ID: 6782999
• Journal Information: Infect. Immun.; (United States), Journal Name: Infect. Immun.; (United States) Vol. 44:2; ISSN INFIB
• Country of Publication: United States
• Language: English

Related Subjects

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550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
MONKEYS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
STAPHYLOCOCCUS
THYMIDINE
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES