Monoclonal antibodies to amyloid subunit proteins for in vivo radioimmunodetection of amyloid diseases

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:6781742

Srivastava, S C; Meinken, G E; Gorevic, P; Atkins, H L; Fand, I; Marshall, J; McNally, W; Muller, D; Wood, D

Amyloid fibrils of systemic amyloidosis are low molecular weight subunit proteins with poor immunogenicity and a tendency to polymerize. Antibodies to these proteins are useful for the detection of amyloid deposits in-situ. The extracellular location of amyloid deposits and proximity to congophilic angiopathy suggest the potential of labeled monocional antibodies (MAb) for in vivo radioimmunodetection. The authors tested feasibility of this approach using two rat MAbs to mouse AA protein in casein-induced amyloidosis, a model system for human secondary amyloidosis. The antibodies were labeled with I-125, I-123, and In-111 with good specificity retention. Amyloidotic mice were pretreated with 50 ..mu..g colchicine ip 3 hr before receiving radioiodinated MAb via the tall vein. Controls included injection of MAb to normal mice and of labeled polyclonal normal rat IgG (pIg) into amyloidotic and control mice. Blood clearance of MAb was faster in amyloidotic than control groups. Fractionation studies showed that both MAb and pIg were uncomplexed. Studies up to 96 hr showed specific and high uptake at sites of amyloid deposition (saline perfused liver, spleen, kidney. Specific localization was confirmed by whole body autoradiography (I-125, 20 ..mu..Ci/animal; 50 ..mu..g MAb) and by external imaging (I-123, 200 ..mu..Ci/animal, 10-15 ..mu..g MAb) of amyloidotic mice studied at 4-72 hr. Amyloidotic animals showed perifollicular localization in spleen, periportal in liver, and glomerular in kidney; scans of controls showed diffuse early washout. These results document the feasibility of using MAbs to fibril subunit proteins for the in vivo detection and therapy of amyloidosis.

• Research Organization: State Univ. of New York, Stony Brook, NY
• OSTI ID: 6781742
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHEMICAL REACTIONS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HOURS LIVING RADIOISOTOPES
HUMAN POPULATIONS
IMMUNOASSAY
IMMUNOLOGY
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MAMMALS
MATERIALS
METABOLIC DISEASES
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
NUTRITIONAL DEFICIENCY
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
POPULATIONS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RETENTION
RODENTS
TRACER TECHNIQUES
USES
VERTEBRATES