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Three radioligands compared for determining cytoplasmic estrogen-receptor content of human breast carcinomas

Journal Article · · Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:6781389
; ; ; ; ;
Researchers compared three radioligands for use in a cytoplasmic estrogen-receptor assay, using pooled cytosol from human breast adenocarcinomas. The estrogen receptor content was determined in vitro by a dextran-coated charcoal method involving a 4-h incubation with and without diethylstilbestrol. Tritiated moxestrol failed to come to equilibrium in 4 h, thereby preventing the use of conventional one-component Scatchard analysis. The use of 125I-labeled estradiol resulted in a higher estimate of estrogen-receptor concentration than that obtained with use of tritiated estradiol. This overestimation was not corrected by Scatchard, double-reciprocal, or Woolf plots, or by two different methods of data analysis: least squared and robust. An underestimation of the specific activity of iodoestradiol with respect to that of tritiated estradiol and an unrecognized second component of nonreceptor binding could explain this disparity.
Research Organization:
Division of Nuclear Medicine, George Washington University Medical Center, Washington, DC
OSTI ID:
6781389
Journal Information:
Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:3; ISSN CLCHA
Country of Publication:
United States
Language:
English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BINDING ENERGY
BODY
CARCINOMAS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
COORDINATION NUMBER
CYTOPLASM
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENERGY
ESTROGENS
GLANDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MAMMARY GLANDS
MAN
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PRIMATES
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RADIOISOTOPES
STEROID HORMONES
VERTEBRATES