Simultaneous measurement of estrogen and progesterone receptors in tumor cytosols with use of /sup 125/I-labeled estradiol and of 3H-R5020

Journal Article · Fri May 01 04:00:00 EDT 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5515840

Thibodeau, S N; Freeman, L; Jiang, N S

We describe a dual-isotope assay for measuring the concentration of estrogen receptors and progesterone receptors in tumor cytosols. The concentrations of these receptors are derived from separate Scatchard-plot analyses of a single dextran-coated charcoal assay that incorporates both radioiodinated estradiol and tritiated R5020 as the labeled ligands. The two isotopes, radioiodine and tritium, are easily measured with a liquid-scintillation counter. The concentrations of estrogen and progesterone receptors derived from the dual-label assay are identical to values derived from the respective single-label assays. Moreover, values obtained from an assay with dextran-coated charcoal that incorporates radioiodinated estradiol are identical to those obtained from an assay in which tritiated estradiol is used as the labeled ligand. The dual-isotope assay requires both less time and less tissue.

• OSTI ID: 5515840
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:5; ISSN CLCHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOASSAY
BODY
CHEMICAL ANALYSIS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ESTROGENS
GLANDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
LIQUID SCINTILLATION DETECTORS
MAMMARY GLANDS
MEASURING INSTRUMENTS
MEASURING METHODS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROGESTERONE
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RADIATION DETECTORS
RADIOISOTOPES
RECEPTORS
SCINTILLATION COUNTERS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
USES