Vitamin D metabolites in human milk

Journal Article · Sat May 01 04:00:00 EDT 1982 · J. Pediatr.; (United States)

DOI:https://doi.org/10.1016/S0022-3476(82)80575-1· OSTI ID:6780944

Weisman, Y; Bawnik, J C; Eisenberg, Z; Spirer, Z

The concentrations of unconjugated 25-OHD, 24, 25(OH)2D, and 1,25(OH)2D were measured in human milk by competitive protein-binding radioassays following successive preparative Sephadex LH-20 chromatography and HPLC. The mean (+/- SE) concentration of 25-OHD was 0.37 +/- 0.03 ng/ml, of 24,25(OH)2D was 24.8 +/- 1.9 pg/ml, and of 1,25(OH)2D was 2.2 +/-0.1 pg/ml. The concentration of 25-OHD3 in milk as determined by HPLC and UV detection at 254 nm was 0.27 +/- 0.08 ng/ml. The milk concentrations of vitamin D metabolites did not correlate with the maternal serum 25-OHD levels. The total amounts of unconjugated vitamin D metabolites correspond to the known low bioassayable vitamin D antirachitic activity in human milk.

• Research Organization: Vitamin Research Laboratory, Ichilov Hospital, Tel Aviv, Israel
• OSTI ID: 6780944
• Journal Information: J. Pediatr.; (United States), Journal Name: J. Pediatr.; (United States) Vol. 100:5; ISSN JOPDA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BODY FLUIDS
CHEMISTRY
CHROMATOGRAPHY
FOOD
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
METABOLISM
METABOLITES
MILK
RADIOASSAY
RADIOCHROMATOGRAPHY
SEPARATION PROCESSES
VITAMIN D
VITAMINS