Detection of serum cardiac myosin light chains in acute experimental myocardial infarction: radioimmunoassay of cardiac myosin light chains. [/sup 125/I]

Journal Article · Fri Dec 01 04:00:00 EST 1978 · Circulation; (United States)

DOI:https://doi.org/10.1161/01.CIR.58.6.1130· OSTI ID:6777189

Khaw, B A; Gold, H K; Fallon, J T; Haber, E

To develop a more specific plasma test for myocardial infarction, antibodies specific for cardiac myosin light chains (CM-LC) were elicited that showed less than 3% cross-reactivity with skeletal muscle light chains. These antibodies were used to develop a radioimmunoassay for CM-LC that had a sensitivity of 20 ng (+- 4 sd; P < 0.001). Normal dog plasma showed no measurable concentrations of CM-LC (n = 6). Plasma samples from 10 dogs with experimental myocardial infarction produced by persistent left anterior descending coronary artery (LAD) occlusion were obtained at 0, 2, 4, 6, 24, 48, and 72 hs. the CM-LC were first detectable in all 10 animals 6 hs after occlusion (97.98 +- 14 ng/ml (mean +- SEM); P < 0.0001). Maximal CM-LC levels were usually obtained between 24 and 48 hs. Sham-operated open chest dogs (0 to 48 hs, n = 3) showed no measurable CM-LC in the plasma samples. Another group of 10 dogs were subjected to 5 hs of LAD occlusion, followed by reperfusion. In four dogs, CM-LC were detectable as early as 1 h after reperfusion (81.88 +- 37.75 ng/ml serum). Sera from all 10 dogs showed elevated levels of CM-LC (199.75 +- 24.0 ng/ml) by 24 hs. Peak CM-LC concentrations were obtained in five dogs at 24 hs (247.0 +- 35.28 ng/ml) and in another dog at 120 hs (245 ng/ml). Histochemical infarct size was determined to be 0.5 to 10% of the left ventricular mass at seven days by triphenyltetrazolium chloride staining. The specificity and sensitivity of this radioimmunoassay for detection of CM-LC, unique proteins to the heart, may be valuable in the diagnosis of myocardial infarction.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6777189
• Journal Information: Circulation; (United States), Journal Name: Circulation; (United States) Vol. 58:6; ISSN CIRCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MYOCARDIAL INFARCTION
MYOSIN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
USES
VERTEBRATES