Radioimmunoassay for quantitation of 2,3,7,8-tetrachlorodibenzofuran

Journal Article · Fri Aug 01 00:00:00 EDT 1980 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac50059a026· OSTI ID:6776790

Luster, M I; Albro, P W; Chae, K; Lawson, L D; Corbett, J T; McKinney, J D

A radioimmunoassay is described for the determination of 2,3,7,8-tetrachlorodibenzofuran (TCDF) in commercial preparations of polychlorinated biphenyls (PCBs) as well as environmental samples including animal tissues. A feature of the assay method is the use of nonionic detergent to solubilize the hydrophobic dibenzofurans in a manner permitting their binding to antibodies. The detection limits of the assay range between 20 pg and 4.0 ng of 2,3,7,8-TCDF. Extensive cross-reactivity studies indicated that the antiserum was fairly specific for TCDF although some cross-reactivity was found to structurally similar compounds. Values for TCDF in various Aroclors and animal tissues by radioimmunoassay correlated with those obtained by gas chromatography-mass spectrometry analysis. The radioimmunoassay is applicable to screening samples in order to minimize the demand for mass spectrometric screening and should be simple enough to be performed in most clinical laboratories. 3 tables, 1 figure.

• Research Organization: National Inst. of Environmental Health Sciences, Research Triangle Park, NC
• OSTI ID: 6776790
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 52:9; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400103* -- Radiometric & Radiochemical Procedures-- (-1987)
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIBODIES
AROMATICS
BIPHENYL
BODY
CHEMICAL ANALYSIS
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
ENVIRONMENTAL MATERIALS
FURANS
GAS CHROMATOGRAPHY
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
ISOTOPE APPLICATIONS
MASS SPECTROSCOPY
MATERIALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
SPECTROSCOPY
TISSUES
TRACER TECHNIQUES