Production and characterization of yeast cytochrome c antibodies; immunological studies of mutants with altered cytochrome c synthesis

Technical Report · Tue Jan 01 04:00:00 EST 1980

OSTI ID:6774065

Matner, R R

Mutations at the structural gene, CYC1, for iso-1-cytochrome c and at the structural gene, CYC7, for iso-2-cytochrome c can reduce the levels of the respective proteins by varying degrees in Saccharomyces cerevisiae. Mutations at two other loci, cyc2 and cyc3, that are unlinked to either of the structural genes, specifically reduced the levels of both iso-cytochromes c. The cyc2 mutations can cause as low as 10 to 20% of the normal level and cyc3 mutations can cause complete deficiencies. We have explored the possiblity that the CYC2 and CYC3 loci code for maturation functions in the biosynthesis of cytochrome c. The approach used to characterize the nature of the cyc2 and cyc3 induced deficiencies of cytochrome c involved four steps. The results were used to propose possible roles for the CYC2 and CYC3 encoded functions. The CYC3 encoded function is hypothesized to be enzymatic heme attachment. CYC2 may code for a protein that binds and transports apo-cytochrome c through the outer mitochondrial membrane and/or enhances the activity of the heme attachment enzyme.

• Research Organization: Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 6774065
• Report Number(s): UR-3490-1922
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CYTOCHROMES
DATA
EXPERIMENTAL DATA
FUNCTIONS
FUNGI
GENE MUTATIONS
GENES
INFORMATION
ISOTOPE APPLICATIONS
MICROORGANISMS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
TRACER TECHNIQUES
YEASTS