Bile fluorescence and some early biological effects in fish as indicators of pollution by xenobiotics

Journal Article · Wed Mar 31 23:00:00 EST 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1897/1552-8618(1993)12[765:BFASEB]2.0.CO;2· OSTI ID:6773426

Britvic, S; Lucic, D; Kurelec, B ^[1]

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1. Ruder Boskovic Inst., Zagreb (Croatia)

Simple measurement of bile fluorescence in fish has been proposed as a means for determining exposure to certain waterborne chemicals. This proposal was based on the data obtained by chemical determination of metabolites of compounds to which fish were exposed. Because exposures to most of these compounds cause some profound biological effects in fish, the authors studied the correlation between the increase in bile fluorescence, the induction of liver benzo[a]pyrene monooxygenase (BaPMO) activity, and the increase in the potential of liver to bioactivate promutagenic benzo[a]pyrene to Salmonella typhimurium TA100 mutagens. Carp exposed to diesel-2 oil, crude oil, or to a flow of polluted river water simultaneously increased severalfold the levels of all three parameters in a dose- and time-dependent manner. During the recovery from exposure, the levels of bile fluorescence and the BaPMO activity declined and by day 15 reached their natural, preexposure levels. The level of bioactivation potential, however, remained at the higher, induced level throughout the recovery period of 15 d. Seven fish species living in polluted Sava River (near Zagreb, Croatia) revealed severalfold increased levels of these parameters, as compared to their levels in fish living in the reference Korana River (near Karlovac, Croatia) or to their levels in control carp. These results give quantitatively new support to the idea of using simple measurements of fluorescence of diluted bile as a rapid and cheap complementary investigative tool for monitoring and assessment studies.

• OSTI ID: 6773426
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 12:4; ISSN ETOCDK; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BACTERIA
BENZOPYRENE
BILE
BIOLOGICAL INDICATORS
BIOLOGICAL MATERIALS
BODY FLUIDS
CONDENSED AROMATICS
DOSE-RESPONSE RELATIONSHIPS
ENERGY SOURCES
ENZYME INDUCTION
ENZYMES
FISHES
FLUORESCENCE
FOSSIL FUELS
FUELS
GENE REGULATION
HYDROCARBONS
LUMINESCENCE
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PETROLEUM
POLLUTION
PROTEINS
SALMONELLA
SALMONELLA TYPHIMURIUM
SENSITIVITY
TIME DEPENDENCE
TOXICITY
VERTEBRATES
WATER POLLUTION