Ozone inhalation stimulates expression of a neutrophil chemotactic protein, macrophage inflammatory protein 2

Journal Article · Thu Apr 01 04:00:00 EST 1993 · Toxicology and Applied Pharmacology; (United States)

DOI:https://doi.org/10.1006/taap.1993.1074· OSTI ID:6773382

Driscoll, K E; Simpson, L; Carter, J; Hassenbein, D; Leikauf, G D ^[1]

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1. Procter Gamble Company, Cincinnati, OH (United States)

Short-term exposure of humans and animals to ozone results in increased lung neutrophils; however, the mechanisms underlying this response are not completely understood. We examined the potential involvement of the neutrophil chemotactic factor, macrophage inflammatory protein 2 (MIP-2), in ozone-induced inflammation. Exposure-response relationships for ozone and MIP-2 expression were characterized by exposing C57B1/6 mice to 0.1-2 ppm ozone for 3 hr and determining lung levels of MIP-2 mRNA 6 hr after exposure. Temporal relationships between ozone and MIP-2 were determined by exposing mice (2 ppm ozone x 3 hr) and characterizing MIP-2 mRNA expression 0, 2, 6, and 24 hr after exposure. Neutrophils in lung lavage fluid were determined in both exposure-response and time course studies. Ozone concentrations > or = 1.0 ppm increased MIP-2 mRNA and this increase corresponded with recruitment of neutrophils. MIP-2 mRNA was increased immediately after ozone exposure and decreased to control levels by 24 hr. To examine the role of direct oxidant effects in ozone-induced MIP-2 expression, alveolar macrophages were exposed in vitro for 4 hr to 10(-10)-10(-5) M hydrogen peroxide and MIP-2 expression was characterized. MIP-2 mRNA levels in lung macrophages were increased by > or = 10(-9) M hydrogen peroxide. In summary, our findings suggest the chemotactic protein MIP-2 may be responsible, at least in part, for ozone-induced increases in lung neutrophils and indicate that direct exposure of alveolar macrophages to an oxidant is sufficient to induce MIP-2 expression.

• OSTI ID: 6773382
• Journal Information: Toxicology and Applied Pharmacology; (United States), Journal Name: Toxicology and Applied Pharmacology; (United States) Vol. 119:2; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DOSE-RESPONSE RELATIONSHIPS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
IN VITRO
INFLAMMATION
KINETICS
LAVAGE
LEUKOCYTES
LUNGS
MACROPHAGES
MAMMALS
MATERIALS
MICE
NEUTROPHILS
ORGANS
OXYGEN COMPOUNDS
OZONE
PATHOLOGICAL CHANGES
PEROXIDES
PHAGOCYTES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES