Effects of in vitro exposure to nitrogen dioxide on human alveolar macrophage release of neutrophil chemotactic factor and interleukin-1

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Environmental Research; (USA)

DOI:https://doi.org/10.1016/S0013-9351(88)80020-3· OSTI ID:5022003

Pinkston, P; Smeglin, A; Roberts, Jr, N J; Gibb, F R; Morrow, P E; Utell, M J ^[1]

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1. Univ. of Rochester School of Medicine and Dentistry, NY (USA)

To evaluate the potential toxic and immunologic effects of nitrogen dioxide (NO{sub 2}) exposure on cells from the lower respiratory tract, normal human alveolar macrophages obtained by bonchoalveolar lavage were exposed to increasing concentrations of NO{sub 2} using an in vitro exposure system. Alveolar macrophages exposed to 5, 10, or 15 ppm NO{sub 2} for 3 hr showed no difference in cell viability when compared to air-exposed macrophages. In addition, the spontaneous release of neutrophil chemotactic factor (NCF) was not changed by NO{sub 2} exposure, nor was there any effect on the ability of alveolar macrophages to release increased amounts of NCF following stimulation with activated zymosan. Furthermore, alveolar macrophages did not spontaneously release interleukin-1 (IL-1) following air or NO{sub 2} exposure. When stimulated with influenza virus both air- and NO{sub 2}-exposed cells released increased amounts of IL-1, but there was no significant difference in the amount of IL-1 released by air- and NO{sub 2}-exposed alveolar macrophages. Thus, although NO{sub 2} exposure is known to incite an inflammatory response in the lower respiratory tract, using the in vitro exposure system described in this study we were unable to demonstrate a direct toxic effect of NO{sub 2} on viability or any NO{sub 2}-induced change in the release of the immunoregulatory molecules NCF and IL-1.

• OSTI ID: 5022003
• Journal Information: Environmental Research; (USA), Journal Name: Environmental Research; (USA) Vol. 47:1; ISSN ENVRA; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
FUNCTIONS
GROWTH FACTORS
IN VITRO
LUNGS
LYMPHOKINES
MACROPHAGES
MAMMALS
MAN
MITOGENS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
POLLUTANTS
POLLUTION
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
SOMATIC CELLS
VERTEBRATES
VIABILITY