Intracellular phosphorylation of vitellogenin in the liver of estrogen-stimulated Xenopus laevis

Journal Article · Fri Apr 10 00:00:00 EST 1981 · J. Biol. Chem.; (United States)

OSTI ID:6771398

Gottlieb, T A; Wallace, R A

A procedure was developed for the preparation of rough and smooth microsomes from small quantities of liver obtained from estrogen-stimulated Xenopus laevis females. Morphological, biochemical, and enzymatic characterization of these fractions indicates that excellent separation of rough microsomes from smooth microsomes is achieved. In addition, pulse-chase experiments demonstrate that rough and smooth microsomes each exhibit the predictable patterns of incorporation characteristic of secretory protein synthesis and intracellular translocation. This procedure was combined with suitable incubation conditions for pulse-chase experiments which demonstrate the subcellular sites of vitellogenin phosphorylation. The data presented indicate that approximately 70% of the phosphate residues are covalently attached to vitellogenin during its intracellular translocation through the smooth microsomes, while the rough microsomes can account for the remainder of the total incorporated phosphate.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6771398
• Journal Information: J. Biol. Chem.; (United States), Vol. 256:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
PHOSPHORYLATION
BIOSYNTHESIS
ESTROGENS
FROGS
HISTOLOGICAL TECHNIQUES
LIVER
MICROSOMES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
GLANDS
HORMONES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
STEROID HORMONES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques