Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteria

Journal Article · Sun May 01 04:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6768894

Schallenberg, J; Moes, M; Truss, M; Reiser, W; Thomm, M; Stetter, K O; Klein, A

The structural genes encoding the four largest subunits of RNA polymerase, A, B', B'', and C, were physically mapped in Methanobacterium thermoautotrophicum Winter. The genes formed a cluster in the order B'', B', A, C and had a common orientation. DNA hybridization experiments yielded different degrees of homology between RNA polymerase gene sequences of different species of Methanobacterium and Methanococcus voltae. No homology was detectable between Methanobacterium thermoautotrophicum and Methanosarcina barkeri. From Southern hybridization experiments in which probes of the four genes from Methanobacterium thermoautotrophicum Winter and restriction digests of the genomic DNAs of the different methanogens were used, a common gene order of the RNA polymerase genes could be deduced.

• Research Organization: Philipps-Universitaet, Marburg/Lahn (West Germany)
• OSTI ID: 6768894
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 170:5; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

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550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CLONING
COMPARATIVE EVALUATIONS
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENETIC MAPPING
HYBRIDIZATION
MAPPING
METHANOGENIC BACTERIA
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RNA POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES