Structure and regulation of methanogen genes: Progress report, July 1, 1987-February 29, 1988
Technical Report
·
OSTI ID:5461296
We are characterizing the histidine biosynthetic genes (hisA and hisI) and methyl reductase (mcrBDCGA) encoding genes from Methanococcus vannielii and a purine biosynthetic gene (purE) and methyl-viologen reducing hydrogenase (mvh) encoding genes from Methanobacterium thermoautotrophicum. The primary structures of most of these genes have been determined. DNA-dependent RNA polymerases purified from M. vannielii and M. thermoautotrophicum have been used in DNA binding-footprinting experiments to identify promoter sequences. Sites of transcription initiation in vivo have been shown to correlate well with the sites identified in vitro as RNA polymerase binding sites. Current work is aimed at determining how expression of these methanogen genes is regulated in vivo. 5 refs.
- Research Organization:
- Ohio State Univ., Columbus (USA). Dept. of Microbiology
- DOE Contract Number:
- FG02-87ER13731
- OSTI ID:
- 5461296
- Report Number(s):
- DOE/ER/13731-T1; ON: DE88006847
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structure and regulation of methanogen genes: Progress report, March 1, 1988--February 15, 1989
Characterization of the organization of the genome of methanogens and development of genetic exchange systems for Methanococcus vannielii: Final report for the period July 1, 1981-June 30, 1987
Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteria
Technical Report
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6378777
Characterization of the organization of the genome of methanogens and development of genetic exchange systems for Methanococcus vannielii: Final report for the period July 1, 1981-June 30, 1987
Technical Report
·
Thu Oct 01 00:00:00 EDT 1987
·
OSTI ID:5964184
Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteria
Journal Article
·
Sun May 01 00:00:00 EDT 1988
· J. Bacteriol.; (United States)
·
OSTI ID:6768894
Related Subjects
09 BIOMASS FUELS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CLONING
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
GENE OPERONS
GENE REGULATION
METHANOGENIC BACTERIA
MICROORGANISMS
PROGRESS REPORT
STRUCTURAL CHEMICAL ANALYSIS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CLONING
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
GENE OPERONS
GENE REGULATION
METHANOGENIC BACTERIA
MICROORGANISMS
PROGRESS REPORT
STRUCTURAL CHEMICAL ANALYSIS