Structure and regulation of methanogen genes: Progress report, July 1, 1987-February 29, 1988
We are characterizing the histidine biosynthetic genes (hisA and hisI) and methyl reductase (mcrBDCGA) encoding genes from Methanococcus vannielii and a purine biosynthetic gene (purE) and methyl-viologen reducing hydrogenase (mvh) encoding genes from Methanobacterium thermoautotrophicum. The primary structures of most of these genes have been determined. DNA-dependent RNA polymerases purified from M. vannielii and M. thermoautotrophicum have been used in DNA binding-footprinting experiments to identify promoter sequences. Sites of transcription initiation in vivo have been shown to correlate well with the sites identified in vitro as RNA polymerase binding sites. Current work is aimed at determining how expression of these methanogen genes is regulated in vivo. 5 refs.
- Research Organization:
- Ohio State Univ., Columbus (USA). Dept. of Microbiology
- DOE Contract Number:
- FG02-87ER13731
- OSTI ID:
- 5461296
- Report Number(s):
- DOE/ER/13731-T1; ON: DE88006847
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
METHANOGENIC BACTERIA
GENE REGULATION
DNA SEQUENCING
DNA-CLONING
GENE OPERONS
PROGRESS REPORT
BACTERIA
CLONING
DOCUMENT TYPES
MICROORGANISMS
STRUCTURAL CHEMICAL ANALYSIS
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