Modulation of benzo(a)pyrene-induced toxicity and mutagenicity by conjugation enzymes in mammalian cells (CHO/HGPRT Assay)

Technical Report · Tue Dec 31 23:00:00 EST 1985

OSTI ID:6768312

Recio, L

The biotransformation of benzo(a)pyrene (BP) catalyzed by the mixed-function oxidase (MFO) system results in numerous metabolites which are cytotoxic and/or mutagenic to mammalian cells. However, these are conjugated with endogenous compounds such as glucuronic acid, sulfate and glutathione (GSH) resulting in detoxication. The effects of three conjugation enzyme systems, UDP-glucuronyltransferases (UDP-GT), sulfotransferases (ST) and glutathione S-transferases (GSHT) on BP-induced cytotoxicity and mutagenicity were studied using the Chinese hamster ovary cells/hypoxanthine-guanine phosphoribosyltransferase (CHO/HGPRT) assay. These studies were done by supplementing a rat-liver homogenate preparation containing MFO system cofactors. The relationship between BP 7,8-diol-9,10-epoxide (BPDE)-DNA adducts and mutagenicity in the CHO/HGPRT assay was also determined. The results of these studies are discussed. 149 refs., 11 figs., 11 tabs.

• Research Organization: Kentucky Univ., Lexington (USA)
• DOE Contract Number: AC05-76OR00033; AC05-84OR21400
• OSTI ID: 6768312
• Report Number(s): DOE/OR/00033-T411; ON: DE88015644
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
CLASSIFICATION
CONDENSED AROMATICS
DAUGHTER PRODUCTS
DETOXIFICATION
HAMSTERS
HYDROCARBONS
ISOTOPES
MAMMALS
MUTAGENS
ORGANIC COMPOUNDS
RATS
RODENTS
SEPARATION PROCESSES
TOXICITY
VERTEBRATES