Comparison of radiolabeled monoclonal antibodies in an in vitro tissue binding assay

Conference · Wed May 01 00:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6762072

Rusckowski, M; Virzi, F; Hnatowich, D J

The authors previously described a simple in vitro assay for evaluating radiolabeled monoclonal antibodies (Abs) as an aid in selecting for clinical trials those with superior tissue binding properties. Tumor or normal tissues as 1 mm cubes are incubated in microtitre-plate wells containing media and indium-111 labeled Abs and, following rinsing, are counted to measure binding. The assay has now been used to evaluate 4 anti-colorectal tumor Abs against 6 human colorectal tumor tissues and normal liver and colon with an additional Ab (anti-PAP) and tissue (CX-1, an established colorectal tumor) as controls. In addition, 4 anti-breast tumor Abs were evaluated against 7 human tumor tissues and normal tissues from 8 organs. Differences in binding of one Ab to different tissues and of different Abs to one tissue were observed in both studies which were usually for larger than the range of repeat measurements. Lower binding was usually observed for the control Ab and for normal tissues. However, increased binding of anti-tumor Abs occasionally occurred in normal tissues especially in spleen and pancreas tissue with anti-breast tumor Abs and in normal colon tissue with one anti-colorectal tumor Ab. A comparison with immunoperoxidase staining in the same tissues with the anti-breast tumor Abs showed a pattern of specificity not dissimilar from that shown by the assay.

• Research Organization: Univ. of Massachusetts Medical Center, Worcester, MA
• OSTI ID: 6762072
• Report Number(s): CONF-850611-; TRN: 87-010555
• Journal Information: J. Nucl. Med.; (United States), Vol. 26:5; Conference: 32. annual meeting of the Society of Nuclear Medicine, Houston, TX, USA, 2 Jun 1985
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
BIOLOGICAL LOCALIZATION
BIOASSAY
LARGE INTESTINE
NEOPLASMS
LIVER
MAMMARY GLANDS
MONOCLONAL ANTIBODIES
LABELLING
RADIOPHARMACEUTICALS
EVALUATION
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MARKERS
COMPARATIVE EVALUATIONS
IN VITRO
INDIUM 111
METABOLISM
RADIOIMMUNOTHERAPY
RETENTION FUNCTIONS
UPTAKE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
GASTROINTESTINAL TRACT
GLANDS
IMMUNOLOGY
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MEDICINE
MINUTES LIVING RADIOISOTOPES
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
REACTION KINETICS
THERAPY
550501* - Metabolism- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics