Platelet labeling and positron emission tomography in the detection of sites of vascular injury

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6760798

Yano, Y; Brennan, K M; Ebbe, S N; Singh, M; Moyer, B R; Carpenter, D A; Mazoyer, E M; Mathis, C A; Budinger, T F

Reports show that diverse types of injury to the arterial wall elicit varying response reactions often including platelet adhesion. The authors have utilized PET and Ga-68 platelets to study mechanically induced endothelial damage in the rabbit aorta. Ga-68 (t1/2=68min) was eluted as 68-Ga-gallium chloride from a Ge-68/Ga-68 stannic oxide/lN HCl generator and purified by ether extraction from 6N HCl. Ga-68 complex with 2-mercaptopyridine-N-oxide (MPO) was formed in the presence of acetate buffer. Ga-68-MPO labeling efficiency (90-98%) was determined by a newly developed HPLC method using C-18 R/P column and methanol:citrate solvent. Separated platelets were then labelled with Ga-68-MPO and imaged with PET. The embolectomy catheter was introduced intrafemorally and pushed cranially 20 cm to the aortic arch. The bulb was inflated and the aorta scraped 3x before removing the catheter and ligating the artery. Evan's blue dye injected 30 minutes before sacrifice distinguished the damaged from undamaged aorta. As seen in gamma counter results and aortic enface autoradiographs there was a 4 fold increase in activity on the damaged aorta. Whole body PET scans showed an increase in activity along the distal aorta. Damage to the endothelial cells in vivo can be caused by several metabolic factors. The achievement of reliable platelet labeling with a positron emitter allows the authors to image the accumulation and turnover of platelets. Validation of the biological integrity after labeling is being done with Ga-67.

• Research Organization: Donner Lab., Univ. CA, Berkeley, CA
• OSTI ID: 6760798
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CHEMICAL PREPARATION
COMPUTERIZED TOMOGRAPHY
DAMAGE
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
EMBOLI
EMISSION COMPUTED TOMOGRAPHY
EVALUATION
GALLIUM 68
GALLIUM ISOTOPES
HEART
HOURS LIVING RADIOISOTOPES
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MATERIALS
METABOLIC DISEASES
NUCLEI
ODD-ODD NUCLEI
ORGANS
POSITRON COMPUTED TOMOGRAPHY
RABBITS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RECOVERY
SYNTHESIS
TESTING
TOMOGRAPHY
VALIDATION
VASCULAR DISEASES
VERTEBRATES