Cloning of genomic loci and chromosomal localization of the human PCTAIRE-1 and -3 protein kinase genes
Journal Article
·
· Genomics; (United States)
- St. Jude Children's Research Hospital, Memphis, TN (United States)
- St. Jude Children's Research Hospital, Memphis, TN (United States) Univ. of Tennessee, Memphis, TN (United States)
Recent studies on the molecular mechanisms controlling the mammalian cell cycle have disclosed a large family of cdc2-related serine/threonine kinases. Among this gene family, the PCTAIRE protein kinases comprise a distinct subfamily of unknown cellular function. To analyze the genomic structure and chromosomal location of the PCTAIRE-1 and -3 genes, the authors isolated human cosmid clones for each gene by screening a human genomic library with murine PCTAIRE cDNA probes. Overlapping clones encompassing approximately 60 kb of genomic DNA were obtained for both PCTAIRE-1 and -3. These clones were confirmed to encode authentic PCTAIRE genes by the detection of exon-intron structures and the coincidence of the nucleotide sequence of exons to that of the published human cDNAs. Using these cosmic clones as probes for FISH analyses, the chromosomal loci for PCTAIRE-1 and PCTAIRE-3 were assigned to bands Xp11 and 1q31-q32, respectively. 18 refs., 2 figs., 1 tab.
- OSTI ID:
- 6758208
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:1; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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