Correlation of cytotoxicity with elimination of iodine-125 from nude mice inoculated with prelabeled human melanoma cells

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · J. Natl. Cancer Inst.; (United States)

OSTI ID:6757146

Lockshin, A; Giovanella, B C; Quian, C; Mendoza, J T; Vardeman, D M; Stehlin, Jr, J S

BRO human melanoma cells were prelabeled in vitro with (125I)5-iodo-2'-deoxyuridine ((125I)IdUrd) and inoculated into NIH-II nude mice ip, im, sc, or iv. Saline or diphtheria toxin (DT), which is selectively toxic to human cells compared to those of mice, was injected, and the loss of 125I from the animals was monitored daily with a whole-body gamma scintillation detector. For most of the inoculation sites DT accelerated the rate of 125I excretion and in all cases was cytotoxic for the inoculated cells as determined by host survival or measurement of visible tumor growth. Differences between the rates of 125I loss for DT-treated mice compared to untreated mice were most evident for cells inoculated ip or im. These results indicate that (125I)IdUrd prelabeling of human tumor cells inoculated in nude mice offers a rapid method for determination of cytotoxicity in vivo.

• Research Organization: Cancer Research Laboratory, St. Joseph Hospital, Houston, TX
• OSTI ID: 6757146
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 73:2; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
CLEARANCE
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MELANOMAS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RODENTS
SURVIVAL TIME
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES