Sensitive fluorescence assays for urokinase using synthetic peptide 4-methoxy-. beta. -naphthylamide substrates

Journal Article · Sat Jul 15 04:00:00 EDT 1978 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(78)90403-7· OSTI ID:6756330

Bigbee, W L; Weintraub, H B; Jensen, R H

Two assays for the plasminogen activator urokinase using peptide fluorogenic substrates are described. N-carbobenzoxy--glycyl-glycyl-L-arginine-4-methoxy-..beta..-naphthylamide (CBZ-Gly-Gly-Arg-4M..beta..NA) can be used in a direct assay that is simple, rapid, and sensitive to as little as 0.5 IU/ml urokinase. Additional sensitivity, to 0.01 IU/ml urokinase, is obtained in a second method that uses plasminogen as the primary substrate followed by a fluorogenic substrate assay employing N-carbobenzoxy-L-ananyl-L-alanyl-L-lysine-4-methoxy-..beta..-naphthylamide (CBZ-Ala-Ala-Lys-4M..beta..NA) as a specific substrate for the activated plasmin. These assays are as sensitive as the best assays presently in use and are simpler to perform. In addition, these assays can readily be used for kinetic analysis of the hydrolytic activity of urokinase or other plasminogen activators.

• Research Organization: Lawrence Livermore Lab., CA
• OSTI ID: 6756330
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 88:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMIDES
BIOASSAY
ENZYMES
FLUORESCENCE
HYDROLASES
IN VITRO
LUMINESCENCE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHORS
PROTEINS
SENSITIVITY
SUBSTRATES
UROKINASE