Mutagenicity testing for RCRA of residuals produced in the conversion of coal. Final report

Technical Report · Fri Jan 01 04:00:00 EST 1982

OSTI ID:6754150

Data from either direct or S-9 activated sample testing in the CHO system does not demonstrate a significant SCE response following exposure to any of the samples tested. Although there is no way of assessing changes which may have occurred during the first 4 months of storage, these liquid samples do not appear to change over time, at least in reference to their direct-acting capacities. Yet the timing of testing relative to sample collection may be critical to other samples. Therefore more recently received liquid samples (2279, 2278) were tested as soon as was possible after delivery. Additional testing of 2278, done at various times after the initial assay, demonstrates the repeatability over time of our direct acting assay system. The success of the S-9 activation dependent assay has been limited to the 1981-82 period. Therefore it is impossible to assess changes in the activation/detoxification-dependent activities of the samples over time. It has also been noted that the activities of the S-9 preparations vary between experiments. This variation may be due to insufficient Araclor induction of liver enzymes in the S-9 fractions, differences in co-factor preparations or inconsistencies in the CHO cell system. Also the high potency of the S-9 mix with cyclophosphamide in contrast to the limited SCE responses of the samples and S-9 mix could be due to differences in metabolism. Since the activation of cyclophosphamide proceeds through hydroxylation and the slurry samples received are largely hydrocarbons (38% coal), one would not expect them to be metabolized in the same manner. In addition, the limited SCE response to samples may be a function of the type of DNA damage induced and the repair capabilities of the CHO cells. It is conceivable that a different test system, i.e. human lymphocyte culture, could aid in elucidating the genotoxic potential of coal processing wastes.

• Research Organization: Pittsburgh Univ., PA (USA)
• DOE Contract Number: AC22-80PC30337
• OSTI ID: 6754150
• Report Number(s): DOE/PC/30337-T1; ON: DE82019766
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
010405* -- Coal
Lignite
& Peat-- Hydrogenation & Liquefaction
016000 -- Coal
Lignite
& Peat-- Health & Safety
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL CULTURES
COAL LIQUEFACTION
DATA
EXPERIMENTAL DATA
HYDROGEN COMPOUNDS
INFORMATION
LIQUEFACTION
LIQUID WASTES
MUTAGEN SCREENING
NUMERICAL DATA
OXYGEN COMPOUNDS
RESIDUES
SCREENING
SLUDGES
SRC PROCESS
SRC-II PROCESS
THERMOCHEMICAL PROCESSES
TOXICITY
WASTE WATER
WASTES
WATER
WATER TREATMENT