Simultaneous quantification of several cholesterol autoxidation and monohydroxylation products by isotope-dilution mass spectrometry

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · Steroids; (USA)

DOI:https://doi.org/10.1016/0039-128X(90)90109-O· OSTI ID:6749551

Breuer, O; Bjoerkhem, I ^[1]

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1. Huddinge Univ. Hospital (Sweden)

An assay based on isotope-dilution mass spectrometry with deuterium-labeled internal standards was developed for simultaneous quantification of cholest-5-ene-3 beta,7 alpha-diol (7 alpha-hydroxycholesterol), cholest-5 beta,6 beta-epoxy-3 beta-ol (cholesterol-5 beta,6 beta-epoxide), cholest-5 alpha,6 alpha-epoxy-3 beta-ol (cholesterol-5 alpha,6 alpha-epoxide), cholest-5-en-7-one-3 beta-ol (7-oxocholesterol), cholestane-3 beta,5 alpha,6 beta-triol, cholest-5-ene-3 beta,25-diol (25-hydroxycholesterol), and cholest-5-ene-3 beta,26-diol (26-hydroxycholesterol) in one single serum sample. Recovery experiments and replicate analyses showed that the assay was sufficiently sensitive, accurate, and precise. The concentrations of the listed compounds in sera from 19 healthy subjects were determined and are presented.

• OSTI ID: 6749551
• Journal Information: Steroids; (USA), Journal Name: Steroids; (USA) Vol. 55:4; ISSN 0039-128X; ISSN STEDA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACCURACY
ANIMALS
CHOLESTEROL
CHROMATOGRAPHY
DEUTERIUM
GAS CHROMATOGRAPHY
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MASS SPECTROSCOPY
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PRIMATES
RADIOASSAY
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
STEROIDS
STEROLS
TRACER TECHNIQUES
VERTEBRATES