Practical radiochromatographic assay for cholesterol epoxide hydrase. [/sup 14/C tracer technique]
Journal Article
·
· Anal. Biochem.; (United States)
A method for the assay of cholesterol epoxide hydrase activity is described. The assay involves the thin-layer chromatographic separation and quantitation of radiolabeled cholestan-3..beta..,5..cap alpha..,6..cap alpha..-epoxide and its major hydration product, cholestan-3..beta..,5..cap alpha..,6..beta..-triol. Radiochromatographic scanning is employed to quantitate the reaction. The procedure is sensitive, rapid, and nondestructive.
- Research Organization:
- Baylor College, Houston, TX
- OSTI ID:
- 6355316
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 94:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
CHOLESTEROL
CHROMATOGRAPHY
ENZYMES
EPOXIDES
EVEN-EVEN NUCLEI
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LYASES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
STEROIDS
STEROLS
TRACER TECHNIQUES
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
CHOLESTEROL
CHROMATOGRAPHY
ENZYMES
EPOXIDES
EVEN-EVEN NUCLEI
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LYASES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
STEROIDS
STEROLS
TRACER TECHNIQUES
YEARS LIVING RADIOISOTOPES