Specific binding of lactoferrin to brush-border membrane: Ontogeny and effect of glycan chain
- Univ. of California, Davis (USA)
Bioavailability of iron from human milk is exceptionally high. It has been suggested that lactoferrin, the major iron-binding protein in human milk, may participate in this high iron bioavailability from milk. The authors examined the interaction of lactoferrin with the intestinal brush-border membrane using the rhesus monkeys as a model. Brush-border membrane vesicles were prepared from monkeys of various ages. Binding studies with {sup 59}Fe-labeled human and monkey lactoferrin were performed to examine interaction of lactoferrin with the brush-border membrane. Specific saturable binding of lactoferrin was found at all ages studied. The dissociation constant for lactoferrin-receptor binding was 9 {times} 10{sup {minus}6} M. In contrast, no binding of serum transferrin or bovine lactoferrin occurred. Removal of fucose from the lactoferrin glycans resulted in a significant decrease in binding. It was concluded that lactoferrin in milk may function in the process of iron absorption through interaction with a small intestinal receptor and that fucosylated glycans on the carbohydrate chain of lactoferrin are necessary for receptor recognition.
- OSTI ID:
- 6749084
- Journal Information:
- American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:4; ISSN 0002-9513; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ADULTS
AGE DEPENDENCE
AGE GROUPS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FOOD
GLOBULINS
GLUCOPROTEINS
GLYCOPROTEINS
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
IRON 59
IRON CHLORIDES
IRON COMPOUNDS
IRON ISOTOPES
ISOTOPES
JUVENILES
KINETICS
LABELLED COMPOUNDS
LACTOFERRIN
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
METALLOPROTEINS
MILK
MONKEYS
NEONATES
NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SACCHARIDES
TRANSITION ELEMENT COMPOUNDS
UPTAKE
VERTEBRATES