Intestinal lactoferrin receptor: presence and specificity during development
As the major iron-binding protein in breast milk, lactoferrin (Lf) has been suggested to play a role in Fe absorption from milk. The authors previous work has validated the use of the Rhesus monkey as a model for studying this role of Lf. They have identified a specific Lf receptor on the brush border (BB) of juvenile Rhesus small intestine (s.i.) which may facilitate Fe uptake into the mucosal cell. In this study the authors examined the presence and specificity of the Lf receptor during development. BB membrane vesicles were prepared from fetal (113 d gestation), infant (3 m), and adult (12 y) Rhesus s.i.; Binding assays were performed by incubating BB vesicles with 59-Fe-Lf and filtering through a 0.22 ..mu..m filter. The fetal and infant tissues were found to possess receptors with a high affinity for Lf. This early ontogeny indicates the importance of the receptor to the infant. Adult s.i. contained Lf receptors in all regions. Since the adult has no dietary intake of Lf, the receptor may play a role in Fe homeostasis via biliary Lf excretion or may simply continue to be expressed throughout life. The receptors were examined for their affinity for purified bovine Lf and human transferrin, both of which are similar in structure to Lf. No binding was found for either, demonstrating the specificity of the receptor for Lf. The presence of the Lf receptor in fetal tissue and its specificity for Lf implies it is essential for adequate Fe nutrition of the suckling infant.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 5322331
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BODY
CARBOHYDRATES
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELEMENTS
EVEN-ODD NUCLEI
EXCRETION
GASTROINTESTINAL TRACT
GLOBULINS
GLOBULINS-BETA
GLUCOPROTEINS
HOMEOSTASIS
INTERMEDIATE MASS NUCLEI
INTESTINES
IRON
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LACTOFERRIN
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
METALLOPROTEINS
METALS
MONKEYS
NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RUMINANTS
SACCHARIDES
SMALL INTESTINE
TRACER TECHNIQUES
TRANSFERRIN
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES