Effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity
To examine the effects of in vivo hydrocortisone sodium succinate (HC) on natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC), 11 normal adults received a single intravenous bolus of 400 mg hydrocortisone. Lymphocytes were tested for NK activity and ADCC using 51chromium (51Cr)-release and single cell cytotoxicity assays against Molt-4 and sensitized RL O leads to target cells, respectively. Four hours after injection, both NK and ADCC activity were transiently increased in the 51Cr-release system. At 4 hours, there was a twofold increase in the relative frequency of potentially cytotoxic target binding cells but the absolute number of these cells did not change. However, the percentage lysis of bound targets at 4 hours was not altered. These data suggest that: 1) lymphocytes participating in NK and ADCC reactions are refractory to the kinetic and functional effects of HC; 2) the increased lytic activity observed at 4 hours is due to a selective depletion of noncytotoxic cells from the circulation; and 3) NK and ADCC activity did not differ in their responses to HC.
- Research Organization:
- Univ. of Florida Coll. of Medicine, Gainesville
- OSTI ID:
- 6748796
- Journal Information:
- Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 27:1; ISSN ARHEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL KILLING
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
CYTOLOGY
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IN VIVO
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PREGNANES
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TOXICITY
TRACER TECHNIQUES