Release of /sup 86/Rb from rat submandibular gland slices: relation to sodium pump activity

Journal Article · Tue May 01 04:00:00 EDT 1984 · Am. J. Physiol.; (United States)

OSTI ID:6748777

Stewart, D J; Laansoo, M; Sen, A K

Slices of rat submandibular gland were preloaded with /sup 86/Rb, an isotope that can substitute for K/sup +/ in the K/sup +/ release process. The efflux of /sup 86/Rb was monitored in a superfusion apparatus that efficiently removed the /sup 86/Rb as it exited from the tissue slices. Carbachol and the calcium ionophore A23187 activated a calcium-dependent increase in /sup 86/Rb efflux. Dibutyryl cGMP had no detectable effect on /sup 86/Rb efflux in contrast to its activation of ouabain-sensitive uptake of /sup 86/Rb observed in an earlier study. The stimulated release of /sup 86/Rb was not dependent on the presence of either sodium or chloride ion. When /sup 86/Rb efflux was stimulated by carbachol, the efflux rate returned toward the basal rate after a few minutes of exposure to carbachol in the medium. If ouabain was then introduced into the superfusate, a large increase in efflux was stimulated. In the absence of carbachol, only a small increase in /sup 86/Rb efflux was stimulated by ouabain. The effect of ouabain indicates that there was a substantial recycling of /sup 86/Rb between the release and uptake processes in the extracellular space of the tissue slice. The significance of this observation is discussed.

• Research Organization: Univ. of Toronto, Ontario
• OSTI ID: 6748777
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 246:5; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ALKALI METALS
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
GLANDS
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LOSSES
MAMMALS
MEMBRANE TRANSPORT
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
POTASSIUM
RADIOISOTOPES
RATS
RESPONSE MODIFYING FACTORS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SALIVARY GLANDS
SODIUM
STIMULATION
STROPHANTHINS
TISSUES
TRACER TECHNIQUES
VERTEBRATES