Inhibition of cell growth in synchronous human hypernephroma cells by recombinant interferon alpha-D and irradiation

Journal Article · Sat Jan 01 04:00:00 EST 1983 · J. Interferon Res.; (United States)

DOI:https://doi.org/10.1089/jir.1983.3.379· OSTI ID:6748643

Chang, A Y; Keng, P C

It has been reported that interferon (IFN) can inhibit cell division on both normal and malignant cells. However, the effect of IFN on the cells at different phases of the cell cycle is still uncertain. In this report, we have studied the cytostatic properties of interferon in each phase of cell cycle by treating synchronous cell population with IFN alone and/or /sup 60/Co x-irradiation. Human hypernephroma cells ACHN (ATCC no. CRL 1611) originally initiated by Dr. Chang and Dr. Hogan were used in the experiments reported here. The cells were grown in monolayer cultures in MEM supplemented with 10% fetal calf serum. The biological properties of exponential growing cells showed an abnormal karyotype, large vacuoles in the cytoplasm, and a doubling time of 24 h. Cells were synchronized into G1, S, and G2 + M phases by centrifugal elutriation. Autoradiography and flow cytometry data indicated that greater than or equal to 95% G1 cells, greater than or equal to 80% S cells, and greater than or equal to 70% G2 + M cells were obtained by this method. A single dose of 10(3) U human interferon alpha (HuIFN-alpha) (Hoffman-LaRoche, recombinant leukocyte D interferon (IFN-alpha D), RO 22-9859) was given to the synchronous cell populations. The cell cycle delay, growth inhibition and cell viability were measured with the Coulter Counter and Channelyzer system, flow cytometry, and colony-forming assay. Preliminary studies showed that IFN inhibited ACHN cell growth to the same extent at each phase of the cell cycle. However, there was no reduction in the clonogenecity of synchronous cells after IFN treatment. The cell age response after single doses of 600 rad /sup 137/CS r-irradiation showed that S phase cells were resistant while both G1 and G2 + M cells were sensitive to radiation. The results of combining treatment of IFN and irradiation showed additional cell-killing effect as compared with irradiation alone.

• Research Organization: Cancer Center, University of Rochester Medical School, Rochester, NY
• OSTI ID: 6748643
• Journal Information: J. Interferon Res.; (United States), Journal Name: J. Interferon Res.; (United States) Vol. 3:4; ISSN JIRED
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CELL CULTURES
CELL CYCLE
CELL DIVISION
CELL FLOW SYSTEMS
COBALT 60
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
GROWTH
INHIBITION
INTERFERON
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
MITOSIS
NUCLEI
ODD-ODD NUCLEI
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
SYNCHRONOUS CULTURES
SYNERGISM
TUMOR CELLS
X RADIATION
YEARS LIVING RADIOISOTOPES