Characterization of a major 31-kilodalton peptidoglycan-bound protein of Legionella pneumophila

Journal Article · Tue May 01 04:00:00 EDT 1990 · Journal of Bacteriology; (USA)

OSTI ID:6746885

Butler, C A; Hoffman, P S ^[1]

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1. Univ. of Tennessee, Memphis (USA)

A 31-kilodalton (kDa) protein was solubilized from the peptidoglycan (PG) fraction of Legionella pneumophila after treatment with either N-acetylmuramidase from the fungus Chalaropsis sp. or with mutanolysin from Streptomyces globisporus. The protein exhibited a ladderlike banding pattern by autoradiography when radiolabeled ((35S)cysteine or (35S)methionine) PG material was extensively treated with hen lysozyme. The banding patterns ranging between 31 and 45 kDa and between 55 and 60 kDa resolved as a single 31-kDa protein when the material was subsequently treated with N-acetylmuramidase. Analysis of the purified 31-kDa protein for diaminopimelic acid by gas chromatography revealed 1 mol of diaminopimelic acid per mol of protein. When outer membrane PG material containing the major outer membrane porin protein was treated with N-acetylmuramidase or mutanolysin, both the 28.5-kDa major outer membrane protein and the 31-kDa protein were solubilized from the PG material under reducing conditions. In the absence of 2-mercaptoethanol, a high-molecular-mass complex (100 kDa) was resolved. The results of this study indicate that a 31-kDa PG-bound protein is a major component of the cell wall of L. pneumophila whose function may be to anchor the major outer membrane protein to PG. Finally, a survey of other Legionella species and other serogroups of L. pneumophila suggested that PG-bound proteins may be a common feature of this genus.

• OSTI ID: 6746885
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:5; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
CHEMISTRY
CYSTEINE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYMES
EVEN-ODD NUCLEI
GLYCOPROTEINS
GLYCOSYL HYDROLASES
HYDROLASES
ISOTOPES
LEGIONELLA PNEUMOPHILA
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANE PROTEINS
METHIONINE
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PORINS
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
THIOLS