Measure of Legionella pneumophila activity in situ
Conference
·
OSTI ID:6682474
Detection of Legionella pneumophila by serogroup-specific fluorescent antibodies was combined with a tetrazolium dye (INT) to measure electron transport activity. The biological uptake and reduction of the INT dye was studied in pure cultures and in natural water samples with respect to temperature. Uptake was complete within 60 minutes. Controls inhibited with formaldehyde demonstrated little activity. Both the in vitro and in situ determinations suggested that the electron transport system of Legionella was active over a temperature range of 25/sup 0/ to 60/sup 0/C.
- Research Organization:
- Du Pont de Nemours (E.I.) and Co., Aiken, SC (USA). Savannah River Lab.; Rensselaer Polytechnic Inst., Troy, NY (USA)
- DOE Contract Number:
- AC09-76SR00001
- OSTI ID:
- 6682474
- Report Number(s):
- DP-MS-80-107; CONF-810313-1
- Country of Publication:
- United States
- Language:
- English
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